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Purpose: To observe the effects of Run Ze Tonic on thyroid hormone, adrenal function, sex hormones and cyclic nucleotide of mice with kidney-yin deficiency. Methods: ICR mice were randomly grouped into normal control group, model group, Run Ze Tonic high dose group, the middle dose group and low dose group, male and female in half.
The kidney-yin deficiency mice models were established by intragastric administration of levothyroxine sodium before intragastric administration of corresponding drugs once a day for 10 consecutive days. One day after the last administration, the effects were observed by measuring weight.
Conclusion: Run Ze Tonic may play the role of nourishing yin and strengthening kidney by adjusting hormone level disorder, adrenal cortex function and balancing energy metabolism in endocrine system.
Early detection of abnormal expression levels of cancer-related genes is crucial for reducing cancer mortality. Each pair of MLPA probes, containing a designed barcode, represents a gene of interest; thus, the use of various dyes to label different genes was avoided. The unique three-base barcode design on the probes, which can be decoded by pyrosequencing, enables individual quantification of the expression levels of six genes.
Moreover, a new carryover contamination prevention system based on the use of restriction endonucleases was developed for PCR-based diagnostic screening assays. For PCR-based diagnostic screening assays, 0.